Abstract
The effects of platelet-derived growth factor (PDGF) on the expression of intercellular adhesion molecule-1 (ICAM-1) as an indicator of cell activation were investigated in cultured human arterial smooth muscle cells (SMC). PDGF-BB and -AB but not -AA at 2-10 ng/ml stimulated ICAM-1 expression at a subconfluent but not a confluent state in a dose-dependent manner. ICAM-1 expression was induced at 2h, reached a plateau at 4h, and continued for at least 24h after stimulation with PDGF. The maximal stimulatory effect of PDGF-BB at 10 ng/ml was comparable to that by optimal concentrations of other cytokines and inflammatory agents. These data suggested that PDGF was a potent stimulator of ICAM-1.
MeSH terms
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Antigens, CD / biosynthesis
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Arteries / drug effects
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Arteries / metabolism*
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Becaplermin
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Cell Adhesion Molecules / biosynthesis*
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Cells, Cultured
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Cytokines / pharmacology*
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Enzyme-Linked Immunosorbent Assay
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Gene Expression / drug effects
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Humans
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Intercellular Adhesion Molecule-1
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Interleukin-1 / pharmacology
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Interleukin-4 / pharmacology
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Kinetics
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Lipopolysaccharides / pharmacology
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Muscle, Smooth, Vascular / drug effects
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Muscle, Smooth, Vascular / metabolism*
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Phospholipases A / pharmacology
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Platelet-Derived Growth Factor / pharmacology*
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Proto-Oncogene Proteins c-sis
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Recombinant Proteins / pharmacology
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Structure-Activity Relationship
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Antigens, CD
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Cell Adhesion Molecules
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Cytokines
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Interleukin-1
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Lipopolysaccharides
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins c-sis
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1
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Becaplermin
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Interleukin-4
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Phospholipases A
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Tetradecanoylphorbol Acetate