Abstract
A polymorphism was detected in the 3' untranslated region of the bovine gamma-S-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele-specific amplification. The two allele-specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism.
MeSH terms
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Alleles
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Animals
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Base Sequence
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Cattle / genetics*
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Crystallins / genetics*
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DNA, Single-Stranded / chemistry
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Electrophoresis, Agar Gel
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Female
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Gene Frequency
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Genetic Markers
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Male
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Pedigree
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Polymerase Chain Reaction / veterinary
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Polymorphism, Genetic*
Substances
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Crystallins
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DNA, Single-Stranded
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Genetic Markers
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Oligonucleotides
Associated data
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GENBANK/L20162
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GENBANK/L20163
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GENBANK/L20164
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GENBANK/L20165
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GENBANK/L20166
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GENBANK/M21095
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GENBANK/S63515
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GENBANK/U10136
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GENBANK/U10137
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GENBANK/U10138
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GENBANK/U10139