Abstract
A chicken liver cDNA for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Lambda ZAP2 phage library. The chicken liver cDNA codes for a protein that has 89.1, 88.4 and 88.0% amino acid identity with the human, rat and bovine liver isoforms, respectively. The kinetic properties of the rat and chicken liver enzymes, purified to homogeneity after expression in E. coli, were different including negative cooperativity for ATP binding and inhibition by Mg2+ for the chicken liver 6-phosphofructo-2-kinase but not for the rat liver kinase. Differences in the beta-loop ATP signature sequences in the chicken and rat liver kinase domains may explain the kinetic differences and represent the major divergence in the evolution of the enzyme from birds to mammals.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Cattle
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Chickens / genetics*
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DNA / chemistry
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DNA / isolation & purification*
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Humans
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Isoenzymes / chemistry
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Isoenzymes / genetics*
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Isoenzymes / metabolism
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Kinetics
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Liver / enzymology*
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Molecular Sequence Data
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Phosphofructokinase-2
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Phosphoric Monoester Hydrolases / chemistry
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Phosphoric Monoester Hydrolases / genetics*
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Phosphoric Monoester Hydrolases / metabolism
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Phosphotransferases (Alcohol Group Acceptor) / chemistry
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Rats
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Regulatory Sequences, Nucleic Acid
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Sequence Homology, Amino Acid
Substances
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Isoenzymes
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Adenosine Triphosphate
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DNA
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Phosphotransferases (Alcohol Group Acceptor)
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Phosphofructokinase-2
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Phosphoric Monoester Hydrolases
Associated data
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GENBANK/S54076
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GENBANK/S55772
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GENBANK/S64629
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GENBANK/S64630
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GENBANK/S64631
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GENBANK/S64632
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GENBANK/S64635
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GENBANK/S64636
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GENBANK/S64637
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GENBANK/U07167