[Quantification of DNA using image analysis on paraffin sections]

Ann Pathol. 1994;14(4):257-60.
[Article in French]

Abstract

With the new softwares that are available, it is now possible to measure the cellular DNA content on Feulgen stained paraffin sections, without enzymatic dissociation of the samples. The main advantage of this technique is to preserve the cellular histological context and to select the right cells that have to be analyzed. The application is of course to obtain information about cancer prognosis, but it can also be very useful in non tumoral pathology. The DNA measurement on paraffin sections can be applied to the diagnosis of triploidy or tetraploïdy in spontaneous abortions without any karyotype.

Publication types

  • English Abstract

MeSH terms

  • DNA / analysis*
  • Humans
  • Image Processing, Computer-Assisted*
  • Microtomy
  • Paraffin Embedding

Substances

  • DNA