Abstract
The regioselective modification of a snake curaremimetic toxin is described. Toxin-alpha from Naja nigricollis was derivatized with an electron-dense maleimido undecagold cluster 5. The cluster-bound obtained toxin 8 has a very high affinity for the cholinergic binding site (Ki = 70 pM) of Torpedo marmorata nicotinic receptor. It is harboring a compact heavy atom core of about 8 A which makes it very useful for electron microscopy experiments.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding Sites
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Cobra Neurotoxin Proteins / chemical synthesis*
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Cobra Neurotoxin Proteins / chemistry
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Cobra Neurotoxin Proteins / metabolism
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In Vitro Techniques
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Molecular Structure
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Nicotinic Antagonists*
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Organogold Compounds
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Organometallic Compounds / chemical synthesis
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Organometallic Compounds / chemistry
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Organometallic Compounds / metabolism
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Receptors, Nicotinic / metabolism
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Torpedo / metabolism
Substances
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Cobra Neurotoxin Proteins
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Nicotinic Antagonists
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Organogold Compounds
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Organometallic Compounds
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Receptors, Nicotinic
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undecagold
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alpha-cobratoxin