Abstract
We used a PCR-based subtraction cloning procedure with Concanavalin A-treated and untreated animal caps from stage 9 Xenopus embryos to search for genes the expression of which is induced during neurogenesis. One of these genes was found to encode a homolog of mammalian HMG 1 and 2, hence named HMG-X. HMG-X mRNA was maternally transmitted, up-regulated in neuroectoderm-derived tissues throughout early development, and eventually down-regulated in all adult tissues examined except ovary. Our data suggest that we have identified a gene for a member of the HMG1/2 family that could have an important role in neurogenesis.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / genetics*
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Central Nervous System / embryology*
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Cloning, Molecular
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DNA, Complementary / analysis
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Down-Regulation
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Ectoderm
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Embryonic Induction
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Gene Expression Regulation, Developmental
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Gene Library
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HMGB1 Protein
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High Mobility Group Proteins / genetics*
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High Mobility Group Proteins / physiology
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Organ Specificity
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RNA, Messenger / biosynthesis
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Up-Regulation
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Xenopus Proteins*
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Xenopus laevis
Substances
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Carrier Proteins
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DNA, Complementary
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HMG-X protein, Xenopus laevis
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HMGB1 Protein
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High Mobility Group Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Xenopus Proteins