Abstract
Human neutrophil-type (MMP-8) and fibroblast-type (MMP-1) interstitial collagenase, and their inhibition by tetracyclines in saliva from patients with recurrent aphthous ulcers (RAU) or aphthae, were studied by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and enzymological analyses. In the salivary specimens obtained from patients with aphthae, collagenase was found in endogenously active form and was predominantly of MMP-8 type. Topical rinsing treatment with chlortetracycline (Aureomycin) alleviated the discomfort caused by the lesions but did not reduce salivary collagenase amounts; however in vitro, doxycycline inhibited salivary collagenase totally.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Child
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Chlortetracycline / administration & dosage
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Chlortetracycline / therapeutic use*
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Collagenases / metabolism
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Doxycycline / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Female
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Fibroblasts / enzymology
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Humans
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Male
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Matrix Metalloproteinase 1
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Matrix Metalloproteinase 8
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Matrix Metalloproteinase Inhibitors*
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Middle Aged
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Mouthwashes / therapeutic use
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Neutrophils / enzymology
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Recurrence
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Saliva / enzymology
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Stomatitis, Aphthous / drug therapy*
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Stomatitis, Aphthous / enzymology*
Substances
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Matrix Metalloproteinase Inhibitors
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Mouthwashes
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Collagenases
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Matrix Metalloproteinase 8
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Matrix Metalloproteinase 1
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Doxycycline
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Chlortetracycline