Crystallization and preliminary X-ray diffraction analysis of the ArsC protein from the Escherichia coli arsenical resistance plasmid, R773

J Mol Biol. 1994 Oct 7;242(5):701-2. doi: 10.1006/jmbi.1994.1618.

Abstract

Diffraction data to 3.0 A resolution were collected on crystals of ArsC protein from the conjugative resistance factor R773 which mediates arsenical resistance in the Gram negative bacterium Escherichia coli. The crystal system is tetragonal, a = 116.2 A, c = 145.0 A and the space group is either P4(1)2(1)2 or P4(3)2(1)2. The most probable range for the contents of the asymmetric unit is four to eight ArsC molecules (M(r) = 15,811).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / chemistry*
  • Adenosine Triphosphatases / isolation & purification
  • Arsenates / pharmacology
  • Arsenite Transporting ATPases
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / isolation & purification
  • Crystallization
  • Crystallography, X-Ray
  • Escherichia coli / chemistry*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Ion Pumps*
  • Multienzyme Complexes*
  • R Factors / genetics*

Substances

  • Arsenates
  • Bacterial Proteins
  • Ion Pumps
  • Multienzyme Complexes
  • Adenosine Triphosphatases
  • Arsenite Transporting ATPases
  • arsenic acid