Genetic exchange in Trypanosoma brucei spp. can occur when two strains are cotransmitted through the tsetse fly vector, but it is non-obligatory and a comparatively rare event. To increase recovery of hybrids, we crossed drug resistant parental strains and selected hybrids by double drug resistance [15]. Analysis of 29 hybrid clones from five separate genetic exchange events shows independent segregation of marker genes and a high frequency of triploidy, both of which phenomena have been observed previously for other trypanosome crosses. However, in addition we provide evidence of genetic recombination involving the tubulin locus. These three observations strongly support the hypothesis that genetic exchange starts with a meiotic division in T. brucei.