Molecular cloning of rat bone morphogenetic protein (BMP) type IA receptor and its expression during ectopic bone formation induced by BMP

Biochem Biophys Res Commun. 1994 Oct 14;204(1):203-9. doi: 10.1006/bbrc.1994.2445.

Abstract

A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library. The rat BMPR-IA cDNA encodes a protein of 532 amino acids with a single transmembrane domain and a putative serine/threonine kinase domain. The overall amino acid sequence identity between the rat and human BMPR-IA was 97%. Reverse transcriptase-polymerase chain reaction analysis revealed that BMPR-IA mRNA was highly expressed in the BMP-induced bone forming tissues throughout the stages tested.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Bone Development* / drug effects
  • Bone Morphogenetic Protein Receptors
  • Bone Morphogenetic Protein Receptors, Type I
  • Bone Morphogenetic Proteins
  • Bone and Bones*
  • Choristoma / metabolism*
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary / isolation & purification
  • Dental Pulp / metabolism*
  • Gene Expression* / drug effects
  • Gene Library
  • Growth Substances / biosynthesis*
  • Humans
  • Molecular Sequence Data
  • Muscular Diseases / metabolism*
  • Polymerase Chain Reaction / methods
  • Proteins / pharmacology*
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics
  • Receptors, Growth Factor*
  • Sequence Homology, Amino Acid

Substances

  • Bone Morphogenetic Proteins
  • DNA Primers
  • DNA, Complementary
  • Growth Substances
  • Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Growth Factor
  • Bmpr1a protein, rat
  • Bone Morphogenetic Protein Receptors
  • Bone Morphogenetic Protein Receptors, Type I

Associated data

  • GENBANK/D38082