The diversity of substances used illegally as growth promoters in meat production requires the development of multi-analyte methods of analysis involving a sample pretreatment step that is as rapid and as easy as possible, followed by a specific and sensitive determination of several residues within the same run. A general strategy for the screening and confirmatory analysis of fifteen artificial anabolic compounds in urine samples is described. It is based on solid-phase extraction on C18 Empore discs and amino-bonded columns followed, after derivatization (trimethylsilyl or methyloxime-trimethylsilyl derivatives), by gas chromatography coupled with collisionally activated dissociation tandem mass spectrometry.