An analysis of the sequence of part of the right arm of chromosome II of S. cerevisiae reveals new genes encoding an amino-acid permease and a carboxypeptidase

Curr Genet. 1994 Jul;26(1):1-7. doi: 10.1007/BF00326297.

Abstract

We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted membrane-spanning domains suggest a conserved structure for these proteins, with 12 trans-membrane domains. YBR1015 shows strong similarities to serine carboxypeptidases; all three residues of the "catalytic triad" typical of this family of enzymes are conserved in the YBR1015 protein. In a preliminary functional analysis we have created a null allele of the YBR1015 gene, and shown that it is not essential for cellular viability.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Transport Systems
  • Carboxypeptidases / genetics*
  • Cathepsin A
  • Chromosome Mapping
  • Chromosomes, Fungal*
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Membrane Transport Proteins / genetics*
  • Molecular Sequence Data
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Amino Acid Transport Systems
  • Fungal Proteins
  • Membrane Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • Carboxypeptidases
  • Cathepsin A
  • PRC1 protein, S cerevisiae
  • serine carboxypeptidase