A region-specific microdissection library for human chromosome 2p23-->p21 and the analysis of an interstitial deletion of 2p21

Cytogenet Cell Genet. 1995;68(1-2):17-8. doi: 10.1159/000133880.

Abstract

A region-specific library of human chromosome 2p23-->p21 was constructed using microdissection and microcloning techniques. Analysis of 94 single-copy microclones from the library showed that 64% were derived from the dissected region. Ten microclones were further mapped to the 2p21 region using a patient with an interstitial deletion of 2p21 and displaying holoprosencephaly, an abnormal embryonic development in midbrain and midface.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CHO Cells
  • Cell Line
  • Chromosome Deletion*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 2*
  • Cloning, Molecular / methods
  • Cricetinae
  • Deoxyribonuclease HindIII
  • Dissection / methods
  • Embryonic and Fetal Development
  • Face / abnormalities
  • Face / embryology
  • Gene Library*
  • Holoprosencephaly / genetics
  • Humans
  • Hybrid Cells
  • Mesencephalon / abnormalities
  • Mesencephalon / embryology
  • Restriction Mapping

Substances

  • Deoxyribonuclease HindIII