Abstract
In this report we document the derivation of pluripotential embryonic stem (ES) cells in the absence of a feeder layer by supplementation of culture media with either ciliary neurotrophic factor or oncostatin M, or with a combination of interleukin-6 (IL-6) plus soluble interleukin-6 receptor (sIL-6R). These factors all activate gp130-associated signaling processes, as does the previously characterized ES cell maintenance factor Differentiation Inhibiting Activity (Leukemia Inhibitory Factor). In particular, the IL-6/sIL-6R complex is thought to act exclusively through gp130. All ES cell lines derived using IL-6/sIL-6R contributed extensively to chimeras and were transmitted through the germline at high frequency. These findings point to a pivotal role for gp130 in ES cell propagation and may be relevant to attempts to derive ES cells from species other than mouse.
MeSH terms
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Animals
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Blastocyst / cytology*
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Blastocyst / drug effects
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Chimera
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Ciliary Neurotrophic Factor
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Culture Media, Serum-Free
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Culture Techniques / methods
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Embryo Transfer
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Female
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Growth Inhibitors / pharmacology
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Humans
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Interleukin-6 / pharmacology*
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Leukemia Inhibitory Factor
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Lymphokines / pharmacology
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Mice
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Nerve Tissue Proteins / pharmacology
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Oncostatin M
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Ovariectomy
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Peptides / pharmacology
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Rats
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Receptors, Interleukin / physiology*
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Receptors, Interleukin-6
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Recombinant Proteins / pharmacology
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Stem Cells / cytology*
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Stem Cells / drug effects
Substances
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Ciliary Neurotrophic Factor
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Culture Media, Serum-Free
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Growth Inhibitors
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Interleukin-6
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LIF protein, human
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Leukemia Inhibitory Factor
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Lif protein, mouse
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Lymphokines
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Nerve Tissue Proteins
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OSM protein, human
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Osm protein, mouse
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Peptides
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Receptors, Interleukin
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Receptors, Interleukin-6
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Recombinant Proteins
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Oncostatin M