Expression of the sarcoplasmic reticulum Ca(2+)-ATPase in yeast

F Centeno; S Deschamps; A M Lompré; M Anger; M J Moutin; Y Dupont; M G Palmgren; J M Villalba; J V Møller; P Falson

doi:10.1016/0014-5793(94)01104-4

Expression of the sarcoplasmic reticulum Ca(2+)-ATPase in yeast

FEBS Lett. 1994 Oct 31;354(1):117-22. doi: 10.1016/0014-5793(94)01104-4.

Authors

F Centeno¹, S Deschamps, A M Lompré, M Anger, M J Moutin, Y Dupont, M G Palmgren, J M Villalba, J V Møller, P Falson, et al.

Affiliation

¹ Département de Biologie Cellulaire et Moléculaire, CEA, Gif sur Yvette, France.

PMID: 7957891
DOI: 10.1016/0014-5793(94)01104-4

Abstract

We describe here an easy system for the production of mg amounts of the rabbit Ca(2+)-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca(2+)-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium-Transporting ATPases / biosynthesis*
Calcium-Transporting ATPases / genetics
Calcium-Transporting ATPases / isolation & purification
Calcium-Transporting ATPases / metabolism
Cloning, Molecular
DNA, Complementary
Gene Expression
Gene Library
Microsomes / metabolism
Muscle, Skeletal / chemistry
Rabbits
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics*
Sarcoplasmic Reticulum / enzymology*
Temperature

Substances

DNA, Complementary
Calcium-Transporting ATPases