Abstract
Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinobacteria / genetics*
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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DNA Nucleotidyltransferases / genetics
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DNA Transposable Elements / genetics*
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Integrases
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Methanol / metabolism
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Molecular Sequence Data
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Plasmids / genetics*
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Recombination, Genetic / genetics*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Viral Proteins*
Substances
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DNA Transposable Elements
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Viral Proteins
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DNA Nucleotidyltransferases
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Integrases
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excisionase
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Methanol