Supplementation of basal mixture or pumpkin seeds snack gave a higher level of inhibitor of crystal formation or aggregation than the control period. Moreover, pumpkin seeds snack showed inhibition effect to a greater extent than basal mixture. In the light of this study, it is suggested that pumpkin seeds snack, a high nutritive mixture, is satisfactory to improve the nutrients and increase the level of inhibitors of crystal formation or aggregation which will subsequently reduce the risk of bladder stone disease in Thailand.