Expression of 5-lipoxygenase in lymphoblastoid B and T cells

Prostaglandins. 1994 Jul;48(1):21-9. doi: 10.1016/0090-6980(94)90093-0.

Abstract

The expression of arachidonate 5-lipoxygenase (5-LOX, arachidonate: oxygen 5-oxidoreductase; EC 1.13.11.34) and the 5-lipoxygenase activating protein (FLAP) genes in lymphoblastoid B and T cell lines was studied at the transcriptional level by reverse transcription-PCR analysis. Two B cell lines, CESS and SKW 6.4, were found to express both 5-LOX and FLAP mRNA. One T cell line, MOLT 4, expressed only FLAP mRNA. Upon stimulation of intact cells by calcium ionophore, B lymphoblastoid cells produced 5, 12 and 15 hydroxyeicosatetraenoic acids as well as leukotriene B4.

Publication types

  • Comparative Study

MeSH terms

  • 5-Lipoxygenase-Activating Proteins
  • Arachidonate 5-Lipoxygenase / biosynthesis*
  • Arachidonate 5-Lipoxygenase / genetics
  • Arachidonic Acid / metabolism
  • B-Lymphocytes / enzymology
  • B-Lymphocytes / metabolism*
  • Base Sequence
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / genetics
  • Cell Line
  • Gene Expression
  • Humans
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • T-Lymphocytes / enzymology
  • T-Lymphocytes / metabolism*
  • Transcription, Genetic

Substances

  • 5-Lipoxygenase-Activating Proteins
  • ALOX5AP protein, human
  • Carrier Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Arachidonic Acid
  • Arachidonate 5-Lipoxygenase