Abstract
The EPH-related transmembrane tyrosine kinases constitute the largest known family of receptor-like tyrosine kinases, with many members displaying specific patterns of expression in the developing and adult nervous system. A family of cell surface-bound ligands exhibiting distinct, but overlapping, specificities for these EPH-related kinases was identified. These ligands were unable to act as conventional soluble factors. However, they did function when presented in membrane-bound form, suggesting that they require direct cell-to-cell contact to activate their receptors. Membrane attachment may serve to facilitate ligand dimerization or aggregation, because antibody-mediated clustering activated previously inactive soluble forms of these ligands.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Line
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Cell Membrane / metabolism*
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DNA-Binding Proteins*
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Ephrin-A1
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Ephrin-B1
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Humans
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Ligands
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Membrane Proteins / chemistry
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Neurons / metabolism
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Phosphorylation
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Proteins / chemistry
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Proteins / metabolism*
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Proto-Oncogene Proteins*
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Receptor Protein-Tyrosine Kinases / metabolism*
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Receptor, EphA5*
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Recombinant Fusion Proteins / metabolism
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Retroviridae Proteins, Oncogenic / metabolism*
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Solubility
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Transcription Factors*
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Transfection
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Tumor Cells, Cultured
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ets-Domain Protein Elk-1
Substances
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DNA-Binding Proteins
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EHK1 ligand
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Ephrin-A1
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Ephrin-B1
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Ligands
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Membrane Proteins
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Proteins
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Proto-Oncogene Proteins
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Recombinant Fusion Proteins
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Retroviridae Proteins, Oncogenic
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Transcription Factors
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ets-Domain Protein Elk-1
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EPHA5 protein, human
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Receptor Protein-Tyrosine Kinases
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Receptor, EphA5