Abstract
A general transcription factor TFIID was purified from rat liver by a sequential column chromatography including organomercurial Sepharose and Sephacryl S-200 chromatographies, which were developed with an acidic buffer (pH5.5). Analyses by SDS-polyacrylamide gel electrophoresis, assay of eluants after renaturation, and immunoblotting showed that isolated TATA factor is an active 75kDa protein complex which contains 36kDa TATA-binding protein.
MeSH terms
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Animals
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Cell Nucleus / metabolism
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Chromatography, Gel
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Chromatography, Ion Exchange
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DNA-Binding Proteins / isolation & purification*
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DNA-Binding Proteins / metabolism
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Electrophoresis, Polyacrylamide Gel
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Liver / metabolism*
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Molecular Weight
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Protein Binding
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Rats
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TATA-Box Binding Protein
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Transcription Factor TFIID
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Transcription Factors / isolation & purification*
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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TATA-Box Binding Protein
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Transcription Factor TFIID
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Transcription Factors