Abstract
The PRINS technique has been used to rapidly detect chromosomes 21 in both metaphases and interphase nuclei. A specific labeling was obtained in preparations from lymphocytes and amniotic fluid cells. The method is based on annealing of specific oligonucleotide primers and subsequent primer extension by a Taq DNA polymerase. PRINS is an interesting alternative to in situ hybridization for cytogenetic diagnosis and physical mapping.
Publication types
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Comparative Study
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Amniocentesis
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Amniotic Fluid / cytology
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Base Sequence
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Chromosomes, Human, Pair 21*
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DNA Primers*
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DNA, Satellite / analysis
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DNA-Directed DNA Polymerase
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Down Syndrome / diagnosis
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Down Syndrome / genetics
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Fetal Diseases / diagnosis
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Humans
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In Situ Hybridization, Fluorescence*
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Lymphocytes / ultrastructure
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Molecular Sequence Data
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Repetitive Sequences, Nucleic Acid
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Sensitivity and Specificity
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Taq Polymerase
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Time Factors
Substances
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DNA Primers
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DNA, Satellite
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Taq Polymerase
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DNA-Directed DNA Polymerase