Abstract
The dose-response relationship of four commercially available barbiturates (methohexitone, pentobarbitone, phenobarbitone and thiopentone) and of their drug-free solutions on the production of oxygen radicals by neutrophils were tested by N-formylmethionyl-leucyl-phenylalanine (FMLP)-induced granulocyte chemiluminescence and in a cell-free chemiluminescence system. Methohexitone had no effect on neutrophil chemiluminescence. Pentobarbitone, phenobarbitone and thiopentone dose-dependently decreased FMLP-induced chemiluminescence and cell-free chemiluminescence. Suppression of neutrophil chemiluminescence by pentobarbitone and phenobarbitone was due to effects of the drug-free solutions and an osmolality greater than 360 mosmol kg-1. Only thiopentone suppressed granulocyte chemiluminescence drug-specifically. The physicochemical properties of commercially available barbiturate preparations and their solutions, as well as free radical scavenging capacity, have to be considered if these preparations are used to evaluate drug-specific effects on the production of oxygen radicals by neutrophils.
MeSH terms
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Barbiturates / administration & dosage
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Barbiturates / chemistry
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Barbiturates / pharmacology*
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Cell-Free System
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Dose-Response Relationship, Drug
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Free Radicals / metabolism
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Granulocytes / drug effects
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Granulocytes / metabolism
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Horseradish Peroxidase / chemistry
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Humans
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Hydrogen Peroxide / chemistry
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Hydrogen-Ion Concentration
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Luminescent Measurements
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Luminol / pharmacology
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Methohexital / administration & dosage
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Methohexital / chemistry
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Methohexital / pharmacology
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
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Neutrophils / drug effects*
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Neutrophils / metabolism
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Osmolar Concentration
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Pentobarbital / administration & dosage
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Pentobarbital / chemistry
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Pentobarbital / pharmacology
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Phenobarbital / administration & dosage
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Phenobarbital / chemistry
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Phenobarbital / pharmacology
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Reactive Oxygen Species / metabolism
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Solutions / chemistry
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Thiopental / administration & dosage
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Thiopental / chemistry
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Thiopental / pharmacology
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Zymosan / pharmacology
Substances
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Barbiturates
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Free Radicals
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Reactive Oxygen Species
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Solutions
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N-Formylmethionine Leucyl-Phenylalanine
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Luminol
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Zymosan
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Hydrogen Peroxide
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Methohexital
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Horseradish Peroxidase
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Pentobarbital
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Thiopental
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Phenobarbital