Overexpression and purification of eukaryotic transcription factors as glutathione-S-transferase fusions in E. coli

Methods Mol Biol. 1994:30:185-97. doi: 10.1385/0-89603-256-6:185.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Escherichia coli / genetics*
  • Gene Expression
  • Genetic Vectors
  • Glutathione Transferase / genetics*
  • Humans
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Sp1 Transcription Factor / genetics
  • Sp1 Transcription Factor / isolation & purification
  • Transcription Factor TFIID
  • Transcription Factors / genetics*
  • Transcription Factors / isolation & purification

Substances

  • DNA, Complementary
  • Recombinant Fusion Proteins
  • Sp1 Transcription Factor
  • Transcription Factor TFIID
  • Transcription Factors
  • Glutathione Transferase