Botryomycosis is a chronic bacterial infection characterized histologically by granules containing bacteria in microabscesses. Although Staphylococcus aureus is the most common causative agent, other bacteria have been reported to cause botryomycosis. Several factors have been hypothesized to be important in the pathogenesis of botryomycosis including foreign bodies, quantity and virulence of bacterial microorganisms and host immunity. We report a case of renal botryomycosis in a 60 year-old woman. The diagnosis was based on clinical findings of Escherichia coli urinary infection, histological findings of granules and immunohistologic findings of anti-Escherichia coli granules staining. This is the seventh reported case of renal botryomycosis.