Abstract
Primers proposed for the diagnosis of the pathogenic spirochete Leptospira spp. (C. Gravekamp, H. V. D. Kemp, M. Franzen, D. Carrington, G.J. Schoone, G.J.J.M. Van Eys, C. O. R. Everard, R.A. Hartskeel, and W.J. Terpstra, J. Gen. Microbiol. 139:1691-1700, 1993) have been found to produce complex serovar-specific patterns under low-stringency PCR conditions. Such patterns obtained by low-stringency PCR, which maintain the specific band as an internal control, offer, an approach to the standardized identification of Leptospira serovars in clinical laboratories.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / standards
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Bacteriological Techniques / statistics & numerical data
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Base Sequence
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DNA Primers / genetics*
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DNA, Bacterial / genetics
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Evaluation Studies as Topic
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Humans
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Leptospira / classification
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Leptospira / genetics*
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Leptospira / isolation & purification*
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Leptospirosis / diagnosis
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / standards
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Polymerase Chain Reaction / statistics & numerical data
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Reproducibility of Results
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Sensitivity and Specificity
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Serotyping
Substances
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DNA Primers
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DNA, Bacterial