Abstract
The TAR element (Tax-responsive element; also called TxRE) is a major determinant of the regulation of bovine leukemia virus (BLV) expression. In order to gain insight into the mechanisms of viral expression, complexes formed between proteins and the TAR enhancer DNA were analyzed by gel retardation assays. We report here that nuclear lysates from ex vivo-isolated B lymphocytes contain proteins that specifically bind to TAR. An antibody directed toward the cyclic AMP-responsive element binding (CREB) protein supershifted a complex (C1) present only in BLV-infected B lymphocytes. The CREB protein thus appears to be a major transcription factor involved in BLV expression in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / physiology
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Base Sequence
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Cattle
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Cyclic AMP / physiology*
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Cyclic AMP Response Element-Binding Protein / metabolism
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DNA-Binding Proteins / metabolism
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Enhancer Elements, Genetic*
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Gene Expression Regulation, Viral*
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Leukemia Virus, Bovine / genetics*
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Molecular Sequence Data
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Nuclear Proteins / metabolism
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Oligodeoxyribonucleotides / metabolism
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Receptors, Cyclic AMP / physiology*
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Regulatory Sequences, Nucleic Acid
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Repetitive Sequences, Nucleic Acid
Substances
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Cyclic AMP Response Element-Binding Protein
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DNA-Binding Proteins
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Receptors, Cyclic AMP
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Cyclic AMP