Scintigraphy using gallium-67 (67Ga) citrate and penvaralent technetium-99m dimercaptosuccinic acid ([99mTc(V)]DMSA) and other radiological examinations were performed in three patients with solitary muscular sarcoidosis who had tumor-like muscular lesions. Although distinction from other invasive soft tissue tumors was difficult using plain and enhanced computed tomography and magnetic resonance imaging, marked uptake of 67Ga and moderate uptake of [99mTc(V)]DMSA were shown at the sites of granulomatous inflammatory lesions of sarcoidosis. Both 67Ga and [99mTc(V)]DMSA scintigraphy could be of value in the diagnosis and detection of distribution of granulomas of sarcoidosis in the soft tissue and in determining the appropriate region for biopsy.