Abstract
Subunit 6 (S6), an integral component of the 26S protease from human erythrocytes, has been studied by SDS-PAGE, peptide mapping and sequence analysis. S6 was cleaved with CNBr and three internal peptides were sequenced. A comparison with known proteins in Genbank revealed that all three S6 peptides match the predicted sequence of TBP7, Tat-binding protein 7. Based on peptide matches covering more than 10% of the TBP7 sequence, and the fact that the migration of S6 on SDS-PAGE is consistent with the estimated molecular mass for TBP7, we conclude that subunit 6 of the 26S protease is TBP7.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Adenosine Triphosphatases*
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Amino Acid Sequence
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Cyanogen Bromide
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Erythrocytes / enzymology
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Humans
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Molecular Sequence Data
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Molecular Weight
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Peptide Hydrolases / chemistry*
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Peptide Mapping
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Proteasome Endopeptidase Complex*
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Transcription Factors / chemistry*
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Transcription Factors / isolation & purification
Substances
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DNA-Binding Proteins
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Transcription Factors
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Peptide Hydrolases
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PSMC4 protein, human
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Proteasome Endopeptidase Complex
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ATP dependent 26S protease
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Adenosine Triphosphatases
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ATPases Associated with Diverse Cellular Activities
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Cyanogen Bromide