Abstract
The effect of wortmannin, a selective phosphatidylinositol 3-kinase inhibitor, on the insulin-like effects of growth hormone in isolated adipocytes from rat was investigated. Wortmannin inhibited both the lipogenic and the antilipolytic effects (IC50 approximately 20 nM) with no effect on [125I]-growth hormone binding to the adipocytes. These data suggest that phosphatidylinositol 3-kinase might play an important role in the insulin-like actions of growth hormone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / drug effects
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Adipocytes / metabolism*
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Androstadienes / pharmacology*
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Animals
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Cells, Cultured
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Glucose / metabolism
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Growth Hormone / antagonists & inhibitors
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Growth Hormone / metabolism
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Growth Hormone / pharmacology*
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Insulin / pharmacology*
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Kinetics
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Lipolysis / drug effects*
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Phosphatidylinositol 3-Kinases
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Phospholipase D / antagonists & inhibitors*
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Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors*
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Rats
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Rats, Sprague-Dawley
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Receptors, Somatotropin / metabolism*
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Triglycerides / biosynthesis*
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Wortmannin
Substances
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Androstadienes
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Insulin
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Receptors, Somatotropin
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Triglycerides
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Growth Hormone
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Phosphatidylinositol 3-Kinases
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Phosphotransferases (Alcohol Group Acceptor)
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Phospholipase D
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Glucose
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Wortmannin