Sequencing and characterization of the kinesin-related genes katB and katC of Arabidopsis thaliana

Plant Mol Biol. 1994 Aug;25(5):865-76. doi: 10.1007/BF00028881.

Abstract

Complementary DNAs of two kinesin-related genes, katB and katC, were isolated from Arabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long alpha-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207-754)), which includes the carboxyl-terminal region of KatC, was expressed in Escherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / analysis
  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary / genetics
  • Escherichia coli / genetics
  • Genes, Plant / genetics*
  • Kinesins / biosynthesis
  • Kinesins / classification
  • Kinesins / genetics*
  • Microtubules / metabolism
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Protein Structure, Secondary
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • Recombinant Proteins
  • Adenosine Triphosphatases
  • KatB protein, Arabidopsis
  • KatC protein, Arabidopsis
  • Kinesins

Associated data

  • GENBANK/D21117
  • GENBANK/D21137
  • GENBANK/D21138