Abstract
Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Adenosine Triphosphatases / biosynthesis
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Adenosine Triphosphatases / genetics*
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Transport
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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DNA, Complementary / genetics
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Endosomal Sorting Complexes Required for Transport
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Mice
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Molecular Sequence Data
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Multigene Family / genetics*
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N-Ethylmaleimide-Sensitive Proteins
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Potassium / metabolism
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RNA, Messenger / analysis
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Recombinant Proteins / biosynthesis
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Repressor Proteins / biosynthesis
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Repressor Proteins / genetics*
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Amino Acid
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Suppression, Genetic
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Tissue Distribution
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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DNA, Complementary
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Endosomal Sorting Complexes Required for Transport
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RNA, Messenger
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Recombinant Proteins
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Repressor Proteins
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Vesicular Transport Proteins
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Adenosine Triphosphatases
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ATPases Associated with Diverse Cellular Activities
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N-Ethylmaleimide-Sensitive Proteins
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Nsf protein, mouse
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Vps4b protein, mouse
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Potassium
Associated data
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GENBANK/U10119
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GENBANK/U10120