Abstract
Our characterization of the Saccharomyces cerevisiae TATA box-binding protein (TBP) has led to the identification of nine specific yeast TBP-associated factors (TAFs) ranging in size from 170 to 25 kDa. The amino acid sequence derived from a purified TAF with an apparent M(r) of 170,000 indicates that yeast Taf170 is encoded by the essential yeast gene MOT1. We describe in this report a series of experiments that demonstrate that the protein encoded by MOT1 is a bona fide yeast TAF and that Taf170 forms a separate complex with TBP distinct from the RNA polymerase II-specific multisubunit transcription factor IID TBP-TAF complex. The significance of this unique TBP-Taf170 complex regarding transcriptional regulation is discussed.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cloning, Molecular
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DNA Helicases / genetics*
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DNA Helicases / isolation & purification
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DNA Helicases / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Electrophoresis, Polyacrylamide Gel
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Precipitin Tests
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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TATA Box
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TATA-Binding Protein Associated Factors*
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TATA-Box Binding Protein
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Transcription Factor TFIID
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Transcription Factors / genetics*
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism
Substances
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DNA-Binding Proteins
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Oligodeoxyribonucleotides
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Saccharomyces cerevisiae Proteins
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TATA-Binding Protein Associated Factors
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TATA-Box Binding Protein
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Transcription Factor TFIID
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Transcription Factors
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Adenosine Triphosphatases
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MOT1 protein, S cerevisiae
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DNA Helicases