Abstract
Bladder washings can be used for simultaneous karyometry and proliferation analysis using MiB-1 as a proliferation marker. We analyzed 42 problem cases with a discordance between cytologic and karyometric classification (QUANTICYT), in which the karyometric classification was based on a combination of a nuclear shape parameter and DNA (2cDI). Moreover, 25 concordant cases were analyzed: 5 normal samples, 6 low-grade tumors and 7 high-grade tumors. All normal samples and all low-grade tumors had labelling indices below 10%, and all high-grade tumors over 10%. For the discordant low-grade tumors, the QUANTICYT classification correlated better with the MiB-1 labelling than the cytologic diagnosis. There was a clear correlation between 2cDI and MiB-1 labelling index. Slightly elevated MiB-1 labelling indices might have some prognostic value.
MeSH terms
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Antibodies, Monoclonal / immunology*
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Antigens, Neoplasm / analysis*
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Antigens, Neoplasm / radiation effects
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Biomarkers, Tumor / analysis*
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Biomarkers, Tumor / radiation effects
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Carcinoma, Transitional Cell / chemistry
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Carcinoma, Transitional Cell / pathology*
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Cell Division
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Cell Nucleus / ultrastructure*
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DNA, Neoplasm / analysis
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Follow-Up Studies
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Frozen Sections
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Humans
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Membrane Proteins / analysis*
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Membrane Proteins / radiation effects
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Microwaves*
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Neoplasm Proteins / analysis*
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Neoplasm Proteins / radiation effects
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Paraffin Embedding
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Protein Denaturation / radiation effects
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Sensitivity and Specificity
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Specimen Handling / methods*
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Therapeutic Irrigation
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Urinary Bladder / chemistry
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Urinary Bladder / pathology*
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Urinary Bladder Neoplasms / chemistry
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Urinary Bladder Neoplasms / pathology*
Substances
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Antibodies, Monoclonal
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Antigens, Neoplasm
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Biomarkers, Tumor
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DNA, Neoplasm
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Membrane Proteins
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Neoplasm Proteins