Cloning and sequencing of the cDNAs encoding two alternative splicing-derived variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor

Biochim Biophys Acta. 1994 Sep 8;1223(2):306-8. doi: 10.1016/0167-4889(94)90241-0.

Abstract

Two distinct cDNA clones, corresponding to alternative mRNA splicing variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor (GM-CSF-R alpha) were isolated from human blood mononuclear cells by RT-PCR. The first one lacks 179 bp, and the second one lacks 136 bp of the major variant, GM-CSF-R alpha 1. Both of variants result in shifts in the reading frame and are expected to encode 377- and 285-amino-acid membrane anchoring and soluble receptor isoforms, respectively.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • DNA, Complementary / isolation & purification
  • Humans
  • Molecular Sequence Data
  • RNA Splicing / genetics
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / chemistry
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics*

Substances

  • DNA, Complementary
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor

Associated data

  • GENBANK/L29348
  • GENBANK/L29349