Lethality and mutagenesis of B lymphocyte progenitor cells following exposure to alpha-particles and X-rays

Int J Radiat Biol. 1994 Aug;66(2):197-205. doi: 10.1080/09553009414551101.

Abstract

B lymphocyte precursor cells are the target cells for the major subtype of paediatric cancer, acute lymphoblastic leukaemia. Using a murine IL-7-dependent clonogenic assay for normal B cell precursors as a model, we have investigated the sensitivity of these cells versus other normal and leukaemic haemopoietic cells to alpha-particle radiation. We find that B cell precursors are remarkably susceptible to the lethal effects of alpha-particles and have a very low probability of surviving a single alpha-track. B cell precursors are also very sensitive to the lethal effects of low LET X-rays. The mutation frequency in a marker gene (HPRT) does not, however, appear to be greater in B cell precursors that survive X-radiation than in other haemopoietic cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alpha Particles*
  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / physiology*
  • B-Lymphocytes / radiation effects*
  • Cell Death
  • Cell Transformation, Neoplastic / genetics
  • Cell Transformation, Neoplastic / radiation effects
  • Energy Transfer
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / physiology*
  • Hematopoietic Stem Cells / radiation effects*
  • Hypoxanthine Phosphoribosyltransferase / genetics
  • Mice
  • Mice, Inbred BALB C
  • Mutagenesis / radiation effects*
  • Mutation
  • Plutonium
  • Radiation Tolerance
  • X-Rays*

Substances

  • Plutonium
  • Hypoxanthine Phosphoribosyltransferase