Host reaction against alginate-polylysine microcapsules containing living cells

J Pharm Pharmacol. 1993 Feb;45(2):121-5. doi: 10.1111/j.2042-7158.1993.tb03695.x.

Abstract

Syngeneic and nude mice were injected intraperitoneally with saline, empty microcapsules, aggregates of tumourogenic MO4 cells, encapsulated non-tumourogenic MO cells and encapsulated MO4 cells. The host reaction 4 days after injection, was evaluated by counting the leucocytes in the peritoneal cavity and the cells sticking to recovered microcapsules. A significantly lower number of peritoneal leucocytes was found in mice injected with empty microcapsules or encapsulated MO cells as compared with encapsulated MO4 cells. Histological evaluation showed a significantly higher number of cells sticking to microcapsules containing cells as compared with empty microcapsules. These observations showed that the cellular host reaction against intraperitoneal implants can be evaluated by counting the leucocytes in the peritoneal lavage and histology of recovered capsules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / toxicity*
  • Animals
  • Capsules
  • Cell Aggregation
  • Female
  • Fibroblasts / drug effects
  • Injections, Intraperitoneal
  • Leukocytes / drug effects
  • Mice
  • Mice, Inbred C3H
  • Mice, Nude
  • Microscopy, Electron
  • Peritoneal Cavity / cytology
  • Polylysine / toxicity*
  • Tumor Cells, Cultured / drug effects

Substances

  • Alginates
  • Capsules
  • Polylysine