Abstract
A radioreceptor assay (RRA) was developed to determine plasma levels of amisulpride. In in vitro binding conditions, the RRA technique was sensitive (11.6 nmol/l) and reliable, with a high degree of precision. The reproducibility of the method through statistical coefficients was < 7%. This method was applied to a pilot pharmacokinetic study in rabbits following single i.v. administration of amisulpride at 10 mg/kg. The results obtained were compared with those obtained by high performance liquid chromatography method (HPLC) in order to assess the validity of this new RRA technique.
MeSH terms
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Amisulpride
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Animals
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Antipsychotic Agents / pharmacokinetics*
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Antipsychotic Agents / pharmacology
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Chromatography, High Pressure Liquid
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Corpus Striatum / drug effects
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Corpus Striatum / metabolism
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Dopamine D2 Receptor Antagonists*
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In Vitro Techniques
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Male
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Rabbits
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Radioligand Assay
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Rats
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Rats, Wistar
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Receptors, Dopamine D2 / metabolism
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Spiperone / pharmacokinetics
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Sulpiride / analogs & derivatives*
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Sulpiride / pharmacokinetics
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Sulpiride / pharmacology
Substances
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Antipsychotic Agents
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Dopamine D2 Receptor Antagonists
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Receptors, Dopamine D2
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Spiperone
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Sulpiride
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Amisulpride