Abstract
Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn superoxide dismutase in vitro, confirming that GroEL can interact with beta-stranded proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / metabolism
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Cattle
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Chaperonin 10
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Chaperonin 60
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Chaperonins
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Escherichia coli / enzymology*
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Gene Expression
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Heat-Shock Proteins / metabolism
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Humans
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Metals / metabolism
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Proteins / metabolism*
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Superoxide Dismutase / biosynthesis*
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Superoxide Dismutase / genetics
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Superoxide Dismutase / metabolism
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Xenopus laevis
Substances
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Bacterial Proteins
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Chaperonin 10
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Chaperonin 60
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Heat-Shock Proteins
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Metals
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Proteins
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Superoxide Dismutase
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Chaperonins