Abstract
The authors describe work in progress at the laboratory in Brescia, Italy, on the application of molecular methods to the diagnosis of leptospirosis. This work includes the following: a) Development of polymerase chain reaction (PCR) assays capable of amplifying specific deoxyribonucleic acid fragments from most Leptospira interrogans strains. b) Development of a microtitre-based assay for rapid detection of PCR-positive samples. c) Characterisation of Leptospira strains through restriction endonuclease analysis of PCR products and amplified fragment length polymorphism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Domestic*
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Antibodies, Bacterial / blood
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Antigens, Bacterial / analysis
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DNA, Bacterial / analysis*
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Italy
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Leptospira interrogans / classification
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Leptospira interrogans / genetics*
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Leptospira interrogans / immunology
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Leptospira interrogans / isolation & purification
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Leptospirosis / diagnosis
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Leptospirosis / veterinary*
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Nucleic Acid Hybridization
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Restriction Mapping
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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DNA, Bacterial