Abstract
1. The localization of alpha-adrenoceptors in the plasma membranes of human kidney were investigated by radioligand binding, using an alpha 1-antagonist, [3H]-bunazosin, and an alpha 2-antagonist, [3H]-rauwolscine. 2. Both the maximum binding (Bmax) and dissociation constant (Kd) of [3H]-bunazosin were greater in the cortex than in the medulla. The Bmax of [3H]-rauwolscine in the medulla was greater than in the cortex. 3. Thus, alpha 1-adrenoceptors appeared to be localized predominantly in the cortex, while the alpha 2-adrenoceptors were mainly present in the medulla of the human kidney.
MeSH terms
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Adrenergic alpha-Antagonists / metabolism
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Adult
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Aged
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Aged, 80 and over
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Binding Sites
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Cell Membrane / chemistry
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Female
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Humans
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Kidney Cortex / chemistry*
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Kidney Cortex / metabolism
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Kidney Medulla / chemistry*
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Kidney Medulla / metabolism
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Male
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Middle Aged
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Quinazolines / metabolism
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Radioligand Assay
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Receptors, Adrenergic, alpha-1 / analysis*
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Receptors, Adrenergic, alpha-1 / metabolism
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Receptors, Adrenergic, alpha-2 / analysis*
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Receptors, Adrenergic, alpha-2 / metabolism
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Yohimbine / metabolism
Substances
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Adrenergic alpha-Antagonists
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Quinazolines
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Receptors, Adrenergic, alpha-1
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Receptors, Adrenergic, alpha-2
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Yohimbine
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bunazosin