Abstract
Circulating immune complexes have been described in both juvenile rheumatoid arthritis and in AIDS. We isolated high-molecular-weight complexes from plasma of children with juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection by sequential gel filtration followed by affinity chromatography on protein A-Sepharose. We demonstrate that 1) mixed IgG-, IgM-, and IgA-bearing immune complexes can be found in both juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection and 2) complexes isolated via affinity chromatography on protein A-Sepharose do not have detectable C4 but activate the classic complement pathway in vitro.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Antigen-Antibody Complex / blood*
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Antigen-Antibody Complex / chemistry
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Antigen-Antibody Complex / isolation & purification
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Arthritis, Juvenile / immunology*
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Child
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Child, Preschool
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Chromatography, Affinity
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Complement C4 / metabolism
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Complement C4b*
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Complement Pathway, Classical
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HIV Infections / congenital
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HIV Infections / immunology*
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Humans
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Immunoglobulin A / blood
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Immunoglobulin G / blood*
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Immunoglobulin M / blood
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In Vitro Techniques
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Infant
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Molecular Weight
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Peptide Fragments / metabolism
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Rheumatoid Factor / blood
Substances
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Antigen-Antibody Complex
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Complement C4
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Immunoglobulin A
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Immunoglobulin G
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Immunoglobulin M
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Peptide Fragments
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Complement C4b
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complement C4d
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Rheumatoid Factor