Estrogen and 1,25-(OH)2-D3 change in rats with osteoporosis induced by dexamethasone (DXM) was observed. The calcium and replenishing Kidney Herbs (RKH) was used in treating osteoporosis. The results shown: The level of estrogen and 1,25-(OH)2-D3 in rats with osteoporosis was lower than that of the normal. Calcium and RKH were effective in treating osteoporosis and they could recover the level of estrogen and 1,25-(OH)2-D3 to normal state, the effect of RKH was better than that of calcium. There was positive correlation between the level of estrogen and 1,25-(OH)2-D3. The treatment of RKH might be an optimal method for osteoporotic treatment.