Abstract
Type III homologies of human fibronectin are generally encoded by two exons, with the exception of the ED-A and ED-B repeats which are encoded by a single exon undergoing alternative splicing. We report that also the type III-9 homology is encoded by a single exon. Further more, RT-PCR analysis, performed on mRNA purified from fetal and adult tissues and from normal and tumor-derived cell types, showed that the III-9 region is not undergoing alternative splicing in all samples tested.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Alternative Splicing
-
Base Sequence
-
Cell Line
-
DNA / blood
-
DNA / isolation & purification
-
DNA / metabolism
-
DNA Primers
-
DNA, Neoplasm / isolation & purification
-
DNA, Neoplasm / metabolism
-
Electrophoresis, Agar Gel
-
Exons*
-
Female
-
Fetus
-
Fibronectins / biosynthesis
-
Fibronectins / genetics*
-
Humans
-
Leukocytes / metabolism
-
Molecular Sequence Data
-
Organ Specificity
-
Polymerase Chain Reaction
-
Pregnancy
-
Repetitive Sequences, Nucleic Acid*
-
Tumor Cells, Cultured
Substances
-
DNA Primers
-
DNA, Neoplasm
-
Fibronectins
-
DNA