The gene encoding the kappa-carrageenase of Alteromonas carrageenovora is related to beta-1,3-1,4-glucanases

Gene. 1994 Feb 11;139(1):105-9. doi: 10.1016/0378-1119(94)90531-2.

Abstract

The nucleotide (nt) and deduced amino acid (aa) sequences are reported for the structural gene cgkA encoding kappa-carrageenase (M(r) 44,412) from the marine bacterium Alteromonas carrageenovora (ATCC 43555), a hydrolase involved in the degradation of kappa-carrageenan. The cgkA gene codes for a protein of 397 aa, with a signal peptide of 25 aa. The enzyme is a new member of family 16 of glycosyl hydrolases, which comprises beta-1,3-1,4-glucanases from various sources and the beta-agarase of Streptomyces coelicolor. It is proposed that residue Glu163 in the kappa-carrageenase from A. carrageenovora and Glu155 in the beta-agarase from S. coelicolor are important for catalysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • Genes, Bacterial*
  • Glycoside Hydrolases / genetics*
  • Gram-Negative Aerobic Bacteria / enzymology*
  • Gram-Negative Aerobic Bacteria / genetics*
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Streptomyces / enzymology
  • Streptomyces / genetics

Substances

  • Bacterial Proteins
  • Glycoside Hydrolases
  • licheninase
  • agarase
  • kappa-carrageenase protein, Alteromonadaceae

Associated data

  • GENBANK/X71620
  • SWISSPROT/P04957
  • SWISSPROT/P07883
  • SWISSPROT/P07980
  • SWISSPROT/P17989
  • SWISSPROT/P23903
  • SWISSPROT/P23904
  • SWISSPROT/P27051
  • SWISSPROT/P29716