Preliminary crystallographic studies of the ligand-binding domain of the thyroid hormone receptor complexed with triiodothyronine

J Mol Biol. 1994 Mar 25;237(2):236-9. doi: 10.1006/jmbi.1994.1224.

Abstract

A truncated, recombinant form of the thyroid hormone receptor, including the hormone binding domain, has been co-crystallized with the hormone T3. The crystals are monoclinic, most likely space group P2, with two molecules per asymmetric unit and cell dimensions a = 63.6 A, b = 80.8 A, c = 100.9 A and beta = 92.1 degrees. The crystals diffract to only medium resolution and decay rapidly in the X-ray beam using laboratory sources. By contrast, high resolution, high-quality data are obtained using synchrotron radiation in conjunction with cryocrystallography.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Binding Sites
  • Crystallography*
  • Receptors, Thyroid Hormone / chemistry*
  • Recombinant Proteins / chemistry
  • Triiodothyronine / chemistry*

Substances

  • Receptors, Thyroid Hormone
  • Recombinant Proteins
  • Triiodothyronine