Abstract
Complementation of defined actinorhodin beta-ketoacyl synthase (KS) mutants by various other KS genes suggested that the ORF1-encoded KS may be relatively generalized in function, whereas the ORF2-encoded KS component may provide specificity in polyketide chain construction. Evidence for differential temporal-spatial expression of the actinorhodin and spore pigment KSs in Streptomyces coelicolor was obtained.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Genes, Bacterial / genetics
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Genes, Bacterial / physiology*
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Genetic Complementation Test
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / metabolism
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Mutation / physiology
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Open Reading Frames / genetics
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Open Reading Frames / physiology*
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Rats
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Streptomyces / enzymology*
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Streptomyces / genetics
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Transformation, Genetic