Abstract
A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
2-Acetylaminofluorene / immunology*
-
Alkaline Phosphatase / immunology*
-
Animals
-
Antibodies, Bispecific / biosynthesis*
-
Antibodies, Bispecific / immunology
-
Antibodies, Bispecific / isolation & purification
-
Cell Line
-
Cells, Cultured
-
DNA / analysis*
-
DNA, Bacterial / analysis
-
Electrophoresis, Agar Gel
-
Enzyme-Linked Immunosorbent Assay
-
Hybridomas / immunology
-
Immunoblotting
-
Immunoglobulin G / immunology
-
Mice
-
Mice, Inbred BALB C
-
Nucleic Acid Hybridization
-
Xanthomonas campestris / genetics
Substances
-
Antibodies, Bispecific
-
DNA, Bacterial
-
Immunoglobulin G
-
DNA
-
2-Acetylaminofluorene
-
Alkaline Phosphatase