Abstract
Orphan receptors of the FTZ-F1-related group of nuclear receptors (xFF1r) were identified in Xenopus laevis by isolation of cDNAs from a neurula stage library. Two cDNAs were found, which encode full length, highly related receptor proteins, xFF1rA and B, whose closet relative known so far is the murine LRH-1 orphan receptor. xFF1rA protein expressed by a recombinant vaccinia virus system specifically binds to FTZ-F1 response elements (FRE; PyCAAGGPyCPu). In cotransfection studies, xFF1rA constitutively activates transcription, in a manner dependent on the number of FREs. The amounts of at least four mRNAs encoding full-length receptors greatly increase between gastrula and early tailbud stages and decrease at later stages. At early tailbud stages, xFTZ-F1-related antigens are found in all nuclei of the embryo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / biosynthesis*
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Carrier Proteins / isolation & purification
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Cattle
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Cloning, Molecular
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DNA, Complementary / metabolism
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DNA-Binding Proteins / biosynthesis*
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Drosophila Proteins
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Drosophila melanogaster / metabolism
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Embryo, Nonmammalian / physiology*
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Fushi Tarazu Transcription Factors
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Gene Expression*
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Globins / genetics
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HeLa Cells
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Homeodomain Proteins
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Humans
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Insect Proteins
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Membrane Proteins / biosynthesis*
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Membrane Proteins / isolation & purification
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Mice
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Phylogeny
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Promoter Regions, Genetic
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism
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Receptors, Cytoplasmic and Nuclear
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Receptors, Steroid / biosynthesis*
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Restriction Mapping
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Steroidogenic Factor 1
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Transcription Factors / biosynthesis*
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Transcription, Genetic*
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Transfection
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Xenopus laevis / embryology
Substances
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Carrier Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Drosophila Proteins
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Fushi Tarazu Transcription Factors
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Homeodomain Proteins
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Insect Proteins
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Membrane Proteins
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Cytoplasmic and Nuclear
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Receptors, Steroid
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Steroidogenic Factor 1
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Transcription Factors
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ftz protein, Drosophila
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nuclear hormone receptor FTZ-F1, Drosophila
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Globins
Associated data
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GENBANK/U05001
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GENBANK/U05003