Abstract
Quantitative analysis of polymerase chain reaction (PCR) amplified HIV-1 DNA or cDNA fragments is attained using an automated system that combines capillary-gel electrophoresis (CGE) for high-efficiency separation and laser-induced fluorescence (LIF) for high-sensitivity detection. This system enables the detection of PCR-amplified multiple target DNA or cDNA in the same tube by a single injection with high precision.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acquired Immunodeficiency Syndrome / microbiology
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Automation
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Base Sequence
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Blotting, Southern
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DNA, Complementary / analysis
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DNA, Viral / analysis*
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Electrophoresis / methods*
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Fluorescence
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HIV Core Protein p24 / genetics
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HIV Envelope Protein gp41 / genetics
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HIV-1 / genetics
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HIV-1 / isolation & purification*
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Humans
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Lasers
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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RNA, Viral / analysis
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Sensitivity and Specificity
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Virion / isolation & purification
Substances
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DNA, Complementary
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DNA, Viral
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HIV Core Protein p24
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HIV Envelope Protein gp41
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RNA, Viral