The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor

Mol Cell Biol. 1994 May;14(5):3310-9. doi: 10.1128/mcb.14.5.3310-3319.1994.

Abstract

We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was demonstrated in vitro and in vivo. This repression appears to be general, as transcription mediated by SP1 and Gal4/VP16 was inhibited by RBP-2N. The protein was purified to near homogeneity from human cells on the basis of its binding to a site present in the promoter of the adenovirus pIX gene. The DNA recognition sequence is 5'-TGGGAAAGAA, which is markedly different from the recombination signal sequence originally identified as the target site for this protein. The sequence of the purified protein is 97% identical with that published for the mouse RBP-2N protein. The reported homolog in Drosophila is Suppressor of Hairless. RBP-2N binding sites are present in a number of cellular and viral promoters, so RBP-2N may have a general role in transcriptional repression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Clone Cells
  • DNA / metabolism*
  • DNA Nucleotidyltransferases / biosynthesis
  • DNA Nucleotidyltransferases / isolation & purification
  • DNA Nucleotidyltransferases / metabolism*
  • Drosophila / genetics
  • Drosophila / metabolism
  • Genes, Immediate-Early
  • HeLa Cells
  • Humans
  • Integrases*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / metabolism*
  • Peptide Fragments / chemistry
  • Peptide Fragments / isolation & purification
  • Plasmids
  • Recombinases
  • Recombination, Genetic*
  • Repressor Proteins / biosynthesis
  • Repressor Proteins / isolation & purification
  • Repressor Proteins / metabolism*
  • Transfection

Substances

  • Oligodeoxyribonucleotides
  • Peptide Fragments
  • RBP-2N protein, human
  • Recombinases
  • Repressor Proteins
  • DNA
  • DNA Nucleotidyltransferases
  • Integrases
  • integron integrase IntI1